Journal: PLoS ONE

Article Title: Experimental Sepsis Impairs Humoral Memory in Mice

doi: 10.1371/journal.pone.0081752

Figure Lengend Snippet: Serum IgM and IgG concentrations 2 or 4 wk after CASP (colon ascendens stent peritonitis) are shown here. C57BL/6 mice were immunized and boosted with OVA at 6 and 9 wk, respectively, and CASP was induced 8 wk after the secondary immunization. Control animals were either untreated, only immunized or only CASP induced. A statistical analysis was performed with the one-way ANOVA and Bonferroni’s post test for selected pairs. n=10-18/group. A single experiment is shown here.

Article Snippet: After thorough washing, bound antibodies were detected with biotin labeled rat-anti-mouse-IgM (BD, Heidelberg, Germany 1 µg/ml) or goat-anti-mouse-IgG (Dianova, Hamburg, Germany 1 µg/ml), peroxidase-labeled Streptavidin (Dianova, Hamburg, Germany 5 µg/ml) and AEC-Substrate (Sigma-Aldrich, Steinheim).

Techniques:

Journal: PLoS ONE

Article Title: Experimental Sepsis Impairs Humoral Memory in Mice

doi: 10.1371/journal.pone.0081752

Figure Lengend Snippet: OVA-specific serum-IgM and -IgG at 2 and 4 wk after CASP (colon ascendens stent peritonitis) are shown here (au = arbitrary units). C57BL/6 mice were immunized and boosted with OVA at 6 and 9 wk, respectively, and CASP was induced 8 wk after the secondary immunization. Control animals were either untreated, only immunized or only CASP induced. A combination of two independent experiments with the same trend is shown here. A statistical analysis was performed with the one-way ANOVA and Bonferroni’s post test for selected pairs. n=10-18/group. *: p<0.05; **: p<0.01.

Article Snippet: After thorough washing, bound antibodies were detected with biotin labeled rat-anti-mouse-IgM (BD, Heidelberg, Germany 1 µg/ml) or goat-anti-mouse-IgG (Dianova, Hamburg, Germany 1 µg/ml), peroxidase-labeled Streptavidin (Dianova, Hamburg, Germany 5 µg/ml) and AEC-Substrate (Sigma-Aldrich, Steinheim).

Techniques: